CytoFLEX Flow Cytometer

The CytoFLEX Flow Cytometer, the first introduction to the CytoFLEX Platform, provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation. Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size. All instruments in the platform utilize CytExpert for CytoFLEX Acquisition and Analysis Software.

  • Violet-Blue-Red (V-B-R) Series, fully activated with 13 colors or as few as four, activate additional channels in the future
  • Includes 13 band pass filters: 450/45, 525/40 (2), 585/42, 610/20 (2), 660/10 (2), 690/50, 712/25, 780/60 (3)
  • For higher throughput applications an optional plate loader module is available and for use in regulated environments the software contains tools for 21 CFR Part 11 compliance
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Description

Quality performance, multiple configurations for real power on your benchtop

The high-performance CytoFLEX system offers scalable detection capabilities for up-to three lasers and 13-color research flow cytometry right on your benchtop.

Ready to run in minutes, not hours with CytoFLEX

The CytoFLEX system is easy to learn, easy to operate and easy to maintain.

Cytoflex flow cytometer

  • Superior sensitivity and resolution for excellent fluorescence and nanoparticle detection
  • Compact, reliable design fits right on your benchtop (<50lbs, 16”x16”x13”)
  • Future-proof system with easy upgrade options
  • Powerful performance with up-to 21 flexible configurations (up-to three Lasers, 13 colors)
  • CytExpert experiment-based software for easy multicolor setup and QC
  • Versatile plug-n-play filters (bandpass-only light collection)
  • Integrated optics for alignment stability
  • Accommodates 12x75mm and microtiter tubes

Sensitivity

An intelligent patent-pending optical design optimizes excitation from up-to three lasers (488nm; 638nm; 405nm) and light collection efficiency. With unique flow cell design and integrated optics, the innovative Wavelength Division Multiplexing (WDM) detection module includes solid-state, high efficiency, low-noise detectors for excellent performance. The WDM’s bandpass-only design guarantees flexibility with easily changeable filters and 4-13 configurations.

Nano particle detection

When equipped with a violet laser, the CytoFLEX can be set up to measure Side Scatter from 405nm for enhanced nanoparticle detection. Mie theory predicts that the scattering cross section of a particle, and thus its scattering intensity, depends on the wavelength of light, the angle of collection, and the size, shape, and refractive index of the particle. All other factors being equal, using a shorter illumination wavelength will result in an increase in scattering cross section, and thus more scattered light. The CytoFLEX offers light scattering parameter measurements from the 488nm laser light, and optionally from the 405nm laser to enhance small particle detection.

Maximize investment in CytoFLEX with custom configuration options

With future-proof upgrade options for up-to 13 colors, the CytoFLEX system evolves as your application needs change. Upgrade your configuration in less than one day knowing that all CytoFLEX instruments have been fully tested across all configuration types.

Excitation Fluorescence Channels Fluorochromes
488nm 525/40 BP FITC, Alexa Fluor 488, CFSE, Fluo-3
585/42 BP PE, PI
610/20 BP ECD, PE-Texas Red, PE-CF594, PI
690/50 BP PC5, PC5.5, PerCP, PerCP-Cy5.5, PI
780/60 BP PC7
638nm 660/20 BP APC, Alex Fluor 647, eFluor 660
712/25 BP APC-Alexa Fluor 700, Alexa Fluor 700
780/60 BP APC-Alexa Fluor 750, APC-Cy7, APC-H7, APC-eFlour 780
405nm 450/45 BP Pacific Blue, V450, eFluor 450, BV421
525/40 BP Krome Orange, AmCyan, V500, BV510
610/20 BP Violet610, BV605, Qdot 605
660/20 BP Violet660, BV650, Qdot 655
780/60 BP Viole780, BV785, Qdot 800

Beckman Coulter Flow Cytometry

Exquisite Sensitivity

  • Unique collection of innovative technologies provide exceptional detection capabilities
  • Large dynamic range to resolve dim and bright populations in the same sample
  • Use violet side scatter to detect nanoparticles

Platform Versatility

  • Purchase the number of parameters needed now
  • Add more detection channels by purchasing an activation key
  • Includes full complement of repositionable bandpass filters, non-standard filters also available for even more flexibility

Low Maintenance

  • Lasers, steering optics and flow cell are all mounted onto a vibration isolated table, maintaining optical alignment
  • Low pressure fluidics system
  • Preventive Maintenance can be completed by the user, no service visit required

Automation Enabled

  • Optional 96-well plate sample loader module
  • API (Application Programming Interface) allows external software to control the instrument
  • For automated sample processing and data acquisition integrate with the Biomek i-Series Instrument

Detection Photodiodes
Resolution <300 nm particles (488 nm side scatter), <80 nm polystyrene particles (405 nm side scatter), rCV 3%
Performance <30 MESF for FITC, <10 MESF for PE
Flow Cell Patent-pending, alignment-free integrated optics quartz flow cell (420 μm x 180 μm ID) design with >1.3 numerical aperture
Signal Processing Digital system with 7-decade display
Power Requirements 100-240 VAC, 50/60 Hz
Item Specifications Referenced B53000

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